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Table 1 Summary of HIV/HA-mediated relative infectivity and HA and NA activity

From: Identification of critical residues of influenza neuraminidase in viral particle release

NA construct Luciferase activityª HA U/ml 4-MUNANA Release (a.u.)
  293T cells A549 cells   
NAH 1.7 (±0.03) × 107 6.9 (±0.5) × 106 64 9.3 (±0.41) × 104
   E119V 8.1 (±2.10) × 105 1.2 (±0.1) × 103 0 8.3 (±0.51) × 103
   N234Y 1.7 (±0.02) × 106 4.3 (±1.00) × 105 16 2.5 (±0.04) × 104
   M241R 2.7 (±0.10) × 105 1.6 (±1.1) × 102 0 4.9 (±0.16) × 102
   G248W 9.1 (±10.3) × 106 7.5 (±1.7) × 105 32 2.8 (±0.11) × 104
   G345R 3.7 (±0.90) × 104 1.3 (±)0.3 × 102 0 4.1 (±0.25) × 102
   I257M 1.8 (±0.009) × 107 4.6 (±0.47) × 106 64 8.3 (±0.29) × 104
   K286E 1.4 (±0.02) × 107 1.5 (±0.27) × 106 32 7.9 (±0.38) × 104
   N234Y/I257M 4.1 (±1.9) × 103 3.5 (±4.7) × 102 0 1.6 (±0.24) × 104
   N234Y/K286E 1.6 (±0.11) × 105 1.1 (±0.22) × 104 0 2.1 (±0.32) × 104
   I257M/K286E 9.8 (±0.29) × 103 1.2 (±0.17) × 104 0 1.0 (±0.10) × 104
   N234Y/I257M/K286E 4.3 (±0.49) × 106 4.7 (±0.56) × 106 8 7.0 (±0.37) × 104
NAA* 6.9 (±1.4) × 105 6.7 (±1.5) × 105 2 1.7 (±0.26) × 103
No NA 1.3 (±0.9) × 103 1.3 (±0.7) × 102 0 4.3 (±0.1) × 102
NAA 1.0 (±0.57) × 104 4.9 (±3.4) × 102 0 6.0 (±0.27) × 102
   V119E 5.4 (±0.6) × 103 7.0 (±6.6) × 102 0 6.7 (±0.31) × 102
   Y234N 1.3 (±0.2) × 104 7.3 (±4.9) × 102 0 4.2 (±0.27) × 102
   R241M 1.2 (±0.2) × 104 9.7 (±5.1) × 102 0 4.4 (±0.12) × 102
   R345G 1.3 (±0.1) × 104 7.8 (±2.2) × 102 0 4.3 (±0.42) × 102
   V119E/Y234N 9.1 (±2.9) × 103 6.9 (±3.9) × 102 0 4.6 (±0.14) × 102
   V119E/R241M 1.1 (±0.3) × 104 5.7 (±1.3) × 102 0 4.0 (±0.04) × 102
   V119E/R345G 1.2 (±0.1) × 104 5.0 (±0.6) × 102 0 4.2 (±0.16) × 102
   Y234N/R241M 7.5 (±3.0) × 103 1.5 (±1.0) × 103 0 4.2 (±0.16) × 102
   Y234N/R345G 8.2 (±2.8) × 103 1.1 (±0.9) × 103 0 4.3 (±0.10) × 102
   R241M/R345G 1.8 (±0.2) × 104 1.7 (±0.8) × 103 0 8.2 (±0.47) × 102
V119E/Y234N/R241M 1.0 (±0.05) × 104 2.8 (±1.2) × 102 0 4.2 (±0.23) × 102
   V119E/Y234N/R345G 1.0 (±0.3) × 104 3.3 (±1.8) × 102 0 4.4 (±0.29) × 102
V119E/R241M/R345G 2.1 (±0.25) × 105 1.1 (±0.1) × 104 0 5.4 (±0.09) × 102
Y234N/R241M/R345G ND ND ND ND
V119E/Y234N/R241M/R345G 2.5 (±0.6) × 106 1.5 (±0.1) × 106 2 2.7 (±0.99) × 103
NAC (NAA 176-230) 1.6 (±0.05) × 107 3.0 (±0.57) × 106 64 5.5 (±0.08) × 104
NAC (NAA 88-230) 5.0 (±0.50) × 105 1.9 (±0.58) × 104 0 2.3 (±0.07) × 104
NAC (NAA 1-230) 1.6 (±0.39) × 105 6.8 (±2.1) × 103 0 4.5 (±0.10) × 103
   S95R 4.3 (±0.07) × 105 1.9 (±0.5) × 104 0 2.1 (±0.12) × 104
   V99I/H100Y 5.9 (±0.6) × 105 2.6 (±0.1) × 104 0 3.1 (±0.03) × 104
   V119E 1.4 (±0.08) × 107 2.1 (±0.1) × 106 16 3.0 (±0.09) × 104
   N146S 1.5 (±0.2) × 105 7.0 (±1.5) × 103 0 1.1 (±0.02) × 104
   H155Y/T157A 3.8 (±0.8) × 105 2.4 (±0.3) × 104 0 2.0 (±0.06) × 104
NAC (NAA 226-470) 1.3 (±0.35) × 104 1.1 (±0.5) × 103 0 4.0 (±0.09) × 102
NAC (NAA 226-362) 1.1 (±0.50) × 104 1.4 (±0.5) × 104 0 4.3 (±0.02) × 102
NAC (NAA 226-301) 1.2 (±0.09) × 105 2.5 (±0.7) × 103 0 1.3 (±0.02) × 103
   Y234N 7.2 (±0.56) × 106 3.6 (±0.3) × 105 32 1.5 (±0.04) × 104
N247D/W248G/Q249L 1.0 (±0.31) × 106 6.2 (±2.6) × 103 0 1.5 (±0.09) × 103
   M257I 7.1 (±1.37) × 106 6.0 (±1.0) × 104 4 1.2 (±0.01) × 104
   V263T/V266I 6.5 (±0.80) × 104 1.1 (±0.6) × 103 0 4.8 (±0.37) × 102
   E269N 2.5 (±0.52) × 105 6.4 (±3.0) × 103 0 1.1 (±0.07) × 103
   Y273S 1.2 (±0.07) × 105 2.9 (±0.6) × 103 0 5.0 (±0.26) × 102
   A284T 1.0 (±0.08) × 105 2.6 (±1.0) × 103 0 6.2 (±0.15) × 102
   E286K 1.8 (±0.004) × 107 2.2 (±0.1) × 106 Und 1.6 (±0.04) × 104
   I287V 6.1 (±0.45) × 105 1.2 (±0.1) × 104 0 1.2 (±0.01) × 103
   T288M 1.6 (±0.06) × 106 6.0 (±0.3) × 104 0 5.2 (±0.18) × 103
  1. α All values are in relative light units (RLU). Standard errors of the means are in parentheses.
  2. ND, not determined
  3. Und, undiluted