Rapid detection of anti-Vaccinia virus neutralizing antibodies

Table 4 Details of oligo nucleotides used in PCR reactions

Oligo name	Oligonucleotide sequence 5'→3'	S/A	Ta (°C)	Tm (°C)
c-myc			60.0
c-myc F	GCCAGAGGAGGAACGAGCT	S		59.4
c-myc R	GGGCCTTTTCATTGTTTTCCA	A		54.2
c-myc TM	F-TGCCCTGCGTGACCAGATCC-T	S		65.9
OPV _12/13 /MPXV 82 protein			62.0
OPV F	GCCAATTGTCTTTCTCTTTTACTGA	S		56.2
OPV R	GAAAACATTTAAGGATGAATCCATCT	A		55.4
OPV TMGB	F-CCTTCTATAGATCTGAGAAT NQF MGB	S		65.5
rpo18/polymerase ^#			60.0
rpo18 F	CTGTAGTTATAAACGTTCCGTGTG	S		50.7
rpo 18 R	TTATCATACGCATTACCATTTCGA	A		47.0
rpo18 TM	F-ATCGCTAAATGATACAGTACCCGAAXTCTCTACT-PH
D8L/IMV membrane protein			60.0
D8L F	GGATGTTCTATATACGGGGATGAGTAG	S		57.9
D8L R	AAAGTTAATAAGGTAGATGACACGTTCT	A		58.5
D8L TM	F-TTCTCATCATCAGAATAAA-NFQ MGB	S		65.1

Y = C or T, B = C or G or T, W = A or T, F = FAM label, T = TAMRA label, TM = hydrolysis probe, TMGB = hydrolysis probe coupled to an MGB moiety; MGB = Minor Groove Binder, NFQ = Non-fluorescent quencher, Tm = Melting temperature (calculated by nearest neighbor method), Ta = Annealing temperature, A = antisense orientation, S = sense orientation, # [7].

ISSN: 1743-422X