Rapid detection of anti-Vaccinia virus neutralizing antibodies

Table 1 Variability and efficiency of OPV_12/13 and c-myc real-time PCR assays.

PCR Assay	copy no.	Variability* C_T		R²**	PCR Efficiency (%) **
		intra-assay	inter-assay
OPV _12/13	10⁶	17.58 ± 0.23	17.42 ± 0.20	0.99	96.00
	10⁵	20.33 ± 0.16	20.55 ± 0.20
	10⁴	24.13 ± 0.15	24.34 ± 0.21
	10³	27.01 ± 0.08	27.42 ± 0.41
	10²	31.22 ± 0.08	31.24 ± 0.38
	10¹	34.42 ± 0.05	34.96 ± 0.47
c-myc	10⁶	19.63 ± 0.04	19.74 ± 0.46	0.99	97.62
	10⁵	23.23 ± 0.28	23.09 ± 0.31
	10⁴	26.40 ± 0.05	26.61 ± 0.29
	10³	29.53 ± 0.02	29.65 ± 0.55
	10²	34.73 ± 0.49	33.76 ± 0.97
	10¹	36.74 ± 0.36	36.39 ± 0.58

* for the determination of the intra-assay variability, plasmid standards (10⁶-10¹ plasmid copies) were measured in triplicate, and for the inter-assay variability, measurements were repeated on 3 consecutive days; ** calculation using the slope of the calibration curves for plasmid standards (10¹-10⁶ plasmid copies); The linear detection range is 10⁶-10¹ copies per run. C_T threshold cycle

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