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Table 1 Variability and efficiency of OPV12/13 and c-myc real-time PCR assays.

From: Rapid detection of anti-Vaccinia virus neutralizing antibodies

PCR Assay copy no. Variability* CT R2** PCR Efficiency (%) **
   intra-assay inter-assay   
OPV 12/13 106 17.58 ± 0.23 17.42 ± 0.20 0.99 96.00
  105 20.33 ± 0.16 20.55 ± 0.20   
  104 24.13 ± 0.15 24.34 ± 0.21   
  103 27.01 ± 0.08 27.42 ± 0.41   
  102 31.22 ± 0.08 31.24 ± 0.38   
  101 34.42 ± 0.05 34.96 ± 0.47   
c-myc 106 19.63 ± 0.04 19.74 ± 0.46 0.99 97.62
  105 23.23 ± 0.28 23.09 ± 0.31   
  104 26.40 ± 0.05 26.61 ± 0.29   
  103 29.53 ± 0.02 29.65 ± 0.55   
  102 34.73 ± 0.49 33.76 ± 0.97   
  101 36.74 ± 0.36 36.39 ± 0.58   
  1. * for the determination of the intra-assay variability, plasmid standards (106-101 plasmid copies) were measured in triplicate, and for the inter-assay variability, measurements were repeated on 3 consecutive days; ** calculation using the slope of the calibration curves for plasmid standards (101-106 plasmid copies); The linear detection range is 106-101 copies per run. CT threshold cycle