Mutation of putative N-Linked Glycosylation Sites in Japanese encephalitis Virus Premembrane and Envelope proteins enhances humoral immunity in BALB/C mice after DNA vaccination

Table 1 pVAX-prME mutant vectors and predicted glycosylation patterns.

Amino acid residues of JEV prME protein targeted for mutagenesis. prME -M1 and prME -M2 are single site mutants, whereas prME -M3 is the double mutant. In all mutants, the Asn codon was replaced by an Ala codon in its N-glycosylation sites (N-X-T/S). Asterisks represent mutated N-glycosylation sites.

ISSN: 1743-422X