Figure 4From: Mutation of putative N-Linked Glycosylation Sites in Japanese encephalitis Virus Premembrane and Envelope proteins enhances humoral immunity in BALB/C mice after DNA vaccinationCytokine detection in splenocyte culture supernatants from vaccinated mice. Splenocyte samples (n = 4 mice per group) isolated from the spleens of vaccinated mice on day 35 after the prime immunization were stimulated with purified JEV particles (10 ng/ml). After 72h, the supernatants were collected to examine the levels of the Th1-type cytokine IFN-γ and the Th2-type cytokine IL-4 using commercially available murine cytokine ELISA kits. Data presented as mean ± standard error for four mice per group.Back to article page