Figure 2

Inhibition of Env/CD4-mediated membrane fusion. β-gal activity was measured after CD4 and Env cells were co-cultured when exposed to A) Bacitracin, B) DNTB, C) UC781 and D) T-20, in different circumstances: (red) CD4 cells were exposed to the compound and co-cultured with Env cells for 24 hours. (green) Env cells were exposed to the compound and co-cultured with CD4 cells for 24 hours. (purple) CD4 cells were exposed to the compound for 30 minutes, washed, and co-cultured with Env cells for 24 hours. (blue) Env cells were exposed to the compound for 30 minutes, washed, and co-cultured with CD4 cells for 24 hours. Percentage values are relative to the positive control (no treatment). The data represent the means ± standard deviations from three separate experiments, each of which was carried out in duplicate.