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Figure 3 | Virology Journal

Figure 3

From: The use of an in vitro microneutralization assay to evaluate the potential of recombinant VP5 protein as an antigen for vaccinating against Grass carp reovirus

Figure 3

Neutralization of GCRV by anti-VP5 polyclonal antibody. a, Microneutralization assay. The anti-VP5 polyclonal antibody was tested for four repeats here (Lane 1-4). 50 TCID50 virus was used for the assay in Lanes 1-4 and Lane 6. Dilution of anti-VP5 serum was as indicated in the figure (Lanes 1-4), with the diluted preimmunized sera served as control sera here (Lane 6). Mock infected cells with diluted control sera served as positive control (Lane 5). b, Virus titration assay. The supernantant of each tested well was subjected for TCID50 assay. The data represents the mean value of the four repeats of tested well for each dilution of anti-VP5; the data of Serum control represents the mean value of the 8 test wells served as positive control, in which the control serum was mixed with 50 TCID50 virus in the microneutralization assay. Standard deviation of each representative data was as indicated in the chart.

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Virology Journal

ISSN: 1743-422X