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Figure 2 | Virology Journal

Figure 2

From: The use of an in vitro microneutralization assay to evaluate the potential of recombinant VP5 protein as an antigen for vaccinating against Grass carp reovirus

Figure 2

Immunoblotting assay of GCRV virions with polyclonal antibody anti-VP5. Mock-infected CIK cell lysate from 105 cells (lane 1), purified GCRV viral particles of 1 μg (lane 2) and GCRV-infected CIK cell lysate from 105 cells (lane 3) were subjected for 10% SDS-PAGE analysis and transferred to PVDF membrane. The first antibody used here was the pooled antisera collected from the immunized mice. Second antibody was the HRP-conjugated goat-anti-mouse (Sigma).

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Virology Journal

ISSN: 1743-422X