Skip to main content
Figure 1 | Virology Journal

Figure 1

From: The use of an in vitro microneutralization assay to evaluate the potential of recombinant VP5 protein as an antigen for vaccinating against Grass carp reovirus

Figure 1

Cloning, expression, purification and immunoblotting assay of rVP. a, the 1% agarose gel electrophoresis of vp5 gene fragment amplified from GCRV genome by RT-PCR. b, 10% SDS-PAGE analysis of induced and purified rVP5. c, Immunoblotting assay of rVP5 with monoclonal antibody against VP5 protein. Second antibody was the HRP-conjugated goat-anti-mouse (Sigma). M, Protein marker; U, total cell extract of uninduced bacterial; I, total cell extract of IPTG-induced bacterial; P, purified rVP5.

Back to article page

Virology Journal

ISSN: 1743-422X