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Figure 5 | Virology Journal

Figure 5

From: Japanese encephalitis virus co-opts the ER-stress response protein GRP78 for viral infectivity

Figure 5

Knocking-down the expression of GRP78 does not affect the replication of JE viral RNA. BHK-21 cells were transfected with siRNA against GRP78 or an irrelevant siRNA (scramble siRNA) for 48 hours. The expression level of GRP78 was measured by Western blot analysis using polyclonal antibody specific to GRP78. Transfected cells were then infected with JEV at an MOI of 1. At 24 hour post-infection, the cell lysates were collected to measure the JEV replication using antibodies specific to NS1 and/or NS5. Two independent replicates are shown for the scramble and GRP78 siRNA conditions

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