Figure 3From: Biological and immunological characterization of recombinant Yellow Fever 17D Viruses expressing a Trypanosoma cruzi Amastigote Surface Protein-2 CD8+T cell epitope at two distinct regions of the genomeExpression of the T. cruzi TEWETGQI epitope by recombinant YF17D/E200/Tc and YF17D/NS2B3/Tc viruses. Indirect immunofluorescence assay of Vero cells infected with recombinant viruses, YF 17DD vaccine virus or not infected (Earle's Medium 199) stained with a mouse polyclonal hyperimmune serum to YF17D (HIYF) or polyclonal antibody directed to the TEWETGQI epitope. Second antibodies were Alexa Fluor 488 goat anti-mouse IgG and Alexa Fluor 546 goat anti-mouse IgG for HIYF (green color) or anti-TEWETGQI (red color) preparations, respectively.Back to article page