From: The nucleotide sequence and genome organization of Plasmopara halstedii virus
PhV | SmV A | |
---|---|---|
Morphology | ||
Virion diameter (stained with 2% uranyl acetate) | 37 nm | 40 nm |
Virion shape | isometric | isometric |
Presence or absence of peplomers | present | present |
Genome | ||
Type of nucleic acid | RNA | RNA |
Strandedness | single-stranded | single-stranded |
Size of genome | 4.3 kb | 5.9 kb |
Number of segments | 2 | 3 |
G+C ratio | RNA1: 45% RNA2: 48% - | RNA1: 51% RNA2: 49% RNA3: 51% |
Size of segments and open-reading frames (ORF) | RNA1: 2793 nt (ORF1) RNA2: 1526 nt (ORF2) - | RNA1: 2928 nt (ORF1a, ORF1b) RNA2: 1981 nt (ORF2) RNA3: 977 nt (no ORF) |
Size and function of ORF RdRp: RNA-dependent RNA polymerase CP: Coat protein | ORF1: 2745 nt (RdRp) - ORF2: 1128 nt (CP) | ORF1a: 2697 nt (RdRp) ORF1b: 870 nt (unknown function) ORF2: 1269 nt (CP) |
Size of 5' untranslated region (5' UTR) | RNA1: 18 nt RNA2: 164 nt | RNA1: 66 nt RNA2: 11 nt |
Size of 3' untranslated region (3' UTR) | RNA1: 30 nt RNA2: 234 nt | RNA1: 165 nt RNA2: 701 nt |
3'-terminal poly(A) tract | Poly(A) tracts are present in RNA1 and RNA2. | Poly(A) tracts are lacking at the 3' ends of all 3 RNA. |
Proteins | ||
Number of structural proteins | 1 | 2 |
Size of structural proteins | SDS-PAGE: 36 kDa ORF2: 40 kDa (375 amino acids) Postproteolytic cleavage: 38 kDa | SDS-PAGE: 43 kDa and 39 kDa ORF2: 45 kDa (422 amino acids) Postproteolytic cleavage: 38 kDa |
CP shows similarities to CP of... | SmV A, Tombusviruses | PhV, Tombusviruses |
Number of non-structural proteins | 1 | 1-2 |
Size of non-structural proteins | ORF1: 914 amino acids (104 kDa) - | ORF1a: 898 amino acids (100 kDa) ORF1b: 289 amino acids (33 kDa) |
RdRp shows similarities to RdRp of... | SmV A, Nodaviruses | PhV, Nodaviruses |
Lipids | ||
Content, character, etc. | none | not determined |