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Table 3 Comparison of Plasmopara halstedii virus (PhV) and Sclerophthora macrospora virus A (SmV A).

From: The nucleotide sequence and genome organization of Plasmopara halstedii virus

 

PhV

SmV A

Morphology

  

Virion diameter (stained with 2% uranyl acetate)

37 nm

40 nm

Virion shape

isometric

isometric

Presence or absence of peplomers

present

present

Genome

  

Type of nucleic acid

RNA

RNA

Strandedness

single-stranded

single-stranded

Size of genome

4.3 kb

5.9 kb

Number of segments

2

3

G+C ratio

RNA1: 45%

RNA2: 48%

-

RNA1: 51%

RNA2: 49%

RNA3: 51%

Size of segments and open-reading frames (ORF)

RNA1: 2793 nt (ORF1)

RNA2: 1526 nt (ORF2)

-

RNA1: 2928 nt (ORF1a, ORF1b)

RNA2: 1981 nt (ORF2)

RNA3: 977 nt (no ORF)

Size and function of ORF

RdRp: RNA-dependent RNA polymerase

CP: Coat protein

ORF1: 2745 nt (RdRp)

-

ORF2: 1128 nt (CP)

ORF1a: 2697 nt (RdRp)

ORF1b: 870 nt (unknown function)

ORF2: 1269 nt (CP)

Size of 5' untranslated region (5' UTR)

RNA1: 18 nt

RNA2: 164 nt

RNA1: 66 nt

RNA2: 11 nt

Size of 3' untranslated region (3' UTR)

RNA1: 30 nt

RNA2: 234 nt

RNA1: 165 nt

RNA2: 701 nt

3'-terminal poly(A) tract

Poly(A) tracts are present in RNA1 and RNA2.

Poly(A) tracts are lacking

at the 3' ends of all 3 RNA.

Proteins

  

Number of structural proteins

1

2

Size of structural proteins

SDS-PAGE: 36 kDa

ORF2: 40 kDa (375 amino acids)

Postproteolytic cleavage: 38 kDa

SDS-PAGE: 43 kDa and 39 kDa

ORF2: 45 kDa (422 amino acids)

Postproteolytic cleavage: 38 kDa

CP shows similarities to CP of...

SmV A, Tombusviruses

PhV, Tombusviruses

Number of non-structural proteins

1

1-2

Size of non-structural proteins

ORF1: 914 amino acids (104 kDa)

-

ORF1a: 898 amino acids (100 kDa)

ORF1b: 289 amino acids (33 kDa)

RdRp shows similarities to RdRp of...

SmV A, Nodaviruses

PhV, Nodaviruses

Lipids

  

Content, character, etc.

none

not determined