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Table 1 Primers used for amplification of virus sequences for the production of hairpin RNAi constructs.

From: Selection of target sequences as well as sequence identity determine the outcome of RNAi approach for resistance against cotton leaf curl geminivirus complex

Construct

Primer

Sequence*

Cloning sites in sense orientation

Cloning sites in antisense orientation

CLCRNAiRepC4/pFGC

AC1-4 SF

AC1-4 SR

AC1-4 ASF

AC1-4 ASR

GTTCCATGG CCCAAACTTCAAGTTCTTC

CAGGGCGCGCC CATCAGTTGTCTCCAAT

CAAGGATCC CCCCAAACGTTTTAAAGTAC

TTCTCTAGA ACTTGATCGAAAGAAGAAGG

Asc I+Nco I

Bam HI+Xba I

CLCRNAiRepTrAPREn/pFGC

AC123 SF

AC123 SR

AC123 ASF

AC123 ASR

CCCCTCGAG GCTACGTTGAAGATTAGGAT

TTCGGCGCGCC TCCTGGTCCCAACAGCAGT

TTCTCTAGA AATCCTGGTCCCAACAGCAGT

CCCCCCGGG GCTACGTTGAAGATTAGGAT

Xho I+Asc I

Xba I+Sma I

CLCRNAiIR/pFGC

IR SF

IR SR

IR ASF

IR ASR

TGACTCGAG TCAATTGGAGACAACTGAT

GTTCCATGG TGAAACTTAGTGCGCAAG

GTTGGATCC TGAAACTTAGTGCGCAAG

TGATCTAGA TCAATTGGAGACAACTGAT

Xho I+Nco I

BamHI+Xba I

  1. * The restriction sites included in primers are underlined.
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Virology Journal

ISSN: 1743-422X