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Figure 3 | Virology Journal

Figure 3

From: Selection of target sequences as well as sequence identity determine the outcome of RNAi approach for resistance against cotton leaf curl geminivirus complex

Figure 3

Southern blot analysis of inoculated Nicotiana benthamiana plants. Southern blots of nucleic acids extracted from the inoculated leaves (panel A) and leaves developing subsequent to inoculation (panel B) were probed with a full-length CLCuMV probe. Samples resulted from plants co inoculated with CLCuMV and either CLCRNAiRepTrAPREn/pFGC (lanes 1 to 5), CLCRNAiRepC4/pFGC (lanes 6 to 10) or CLCRNAiIR/pFGC (lanes 11 to 15). The sample in lane 16 was extracted from a symptomatic plant inoculated with only CLCuMV. The positions of single-stranded (ss), super-coiled (sc), linear (lin) and open-circular (oc) viral DNA forms are indicated with arrows.

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Virology Journal

ISSN: 1743-422X