Table 1 Primers for (A): PCR amplification and RT-PCR analysis of the viral genes; (B): RT-PCR analysis of chicken cytokines
Gene | Accession Number | Sequence (5' to 3') | Expected product size (base pair) | |
|---|---|---|---|---|
A: | ||||
CAV VP1 | AF285882 | Forward Reverse | CTAACGCGGCCGCACCATGGCAAGACGAGCTCGC CTAGGGGTACCCCAGTACATGGTGCTGTTGG | 1335 |
CAV VP2 | AF285882 | Forward Reverse | GCTAAGTCGACACCATGCACGGGAACGGC CATGGGGATCCCACTATACGTACCGGGGC | 648 |
MDV-1 VP22 | AY311498 | Forward Reverse | CATGGGGTACCATGGGGGATTCTGAAAGGC GTACGCTCGAGTCGCTATCACTGCTACGAT | 728 |
B: | ||||
IL-4 | NM_001007079 | Forward Reverse | AGCTCTCAGTGCCGCTGATG TAGCTAGTTGGTGGAAGAAGG | 321 |
IL-6 | NM_204628 | Forward Reverse | ATGAACTTCACCGAGGGCTGC ACGGTCTTCTCCATAAACGAAG | 680 |
IL-12 | AY262752 | Forward Reverse | ACACATCTGATGAAGCACTGCC TTGGGATATGTCCAGGTC-ACAG | 598 |
β-actin (positive control) | EU931581 | Forward Reverse | ATGTGCAAGGCCGGTTTCGC TCCTCAGGGGCTACTCTCAG | 254 |