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Figure 5 | Virology Journal

Figure 5

From: Induction of Cyclooxygenase-2 Expression by Hepatitis B Virus Depends on Demethylation-associated Recruitment of Transcription Factors to the Promoter

Figure 5

DNMT3B is responsible for the hypomethylation of COX-2 promoter. (A) DNMT activities of both total cellular and nuclear extracts from HepG2 and HepG2.2.15 cells were measured using the Epiquik DNA Methyltransferase activity assay kit (*P < 0.05). (B) HepG2 cells were transfected with pCOX-2-Luc, pRL-TK, siDNMT1, siDNMT3A or siDNMT3B. Luciferase activites were measured after serum starvation for 24 hours, and all data were normalized to the Renilla luciferase value, and are presented as mean ± SD, n = 3 (*P < 0.05). (C) HepG2 cells were transfected with pCOX-2-Luc, pCDNA-DNMT1, pCDNA-DNMT3A or pCDNA-DNMT3B. Luciferase activites were measured as in (B). (D) HepG2 or HepG2.2.15 cells and HepG2 cells transfected with pHBV-1.2 or control vector were used in ChIP assays using 2 μg anti-DNMT1, -DNMT3A, or -DNMT3B. Immunoprecipitated DNA or control DNA was collected and amplified using specific primers for the COX-2 promoter (nucleotides -177 to -56).

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Virology Journal

ISSN: 1743-422X