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Figure 2 | Virology Journal

Figure 2

From: Induction of Cyclooxygenase-2 Expression by Hepatitis B Virus Depends on Demethylation-associated Recruitment of Transcription Factors to the Promoter

Figure 2

C/EBPβ plays a role in HBx-induced COX-2 promoter activation. (A) HepG2 cells were transfected with luciferase reporter plasmids containing the wild-type COX-2 promoter (-891/+9), or with plasmids containing deletion mutants of the COX-2 promoter, along with pCMV-HBx and pRL-TK. Luciferase activites were measured in each sample after serum starvation for 24 hours, and were compared to those obtained from control cells transfected with empty vector. All data were normalized to the Renilla luciferase value and are presented as mean ± SD, n = 3. (B) HepG2 cells were transfected with luciferase reporter plasmids containing the wild-type COX-2 promoter, or with those containing point mutations in the NFκB, C/EBP and CRE binding sties in the COX-2 promoter, along with pCMV-HBx and pRL-TK. Luciferase activities were measured as described in (A) (*P < 0.05). (C) HepG2 cells were transfected with pCOX-2-Luc, pCMV-HBx, pCMV-LAP, and pRL-TK, and luciferase activity was measured as described in (A) (*P < 0.05). (D) HepG2 cells were transfected with pCOX-2-Luc, pCMV-HBx, pCMV-LIP and pRL-TK, and luciferase activity was measured as described in (A) (*P < 0.05).

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