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Figure 2 | Virology Journal

Figure 2

From: Identification of a mutated BHK-21 cell line that became less susceptible to Japanese encephalitis virus infection

Figure 2

JEV binding to 3A10-3F and BHK-21 cells measured by indirect immunofluorescence assay (IFA). JEV antigen were detected with anti-JEV mAb and FITC-conjugated goat anti-mouse IgG at 24 hr post-infection, and uninfected 3A10-3F cells (a) and BHK-21 cells (c) were negative controls. The cytoplasm and nuclei were stained red by Evans blue, and green fluorescent signals of viral antigen were significantly weaker in 3A10-3F cells (b) than in BHK-21 cells (d) (Magnification: ×400).

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