Skip to main content
Figure 1 | Virology Journal

Figure 1

From: Identification of a mutated BHK-21 cell line that became less susceptible to Japanese encephalitis virus infection

Figure 1

Selection of mutated cell lines negative of JEV E gene RNA fragments by RT-PCR. Eight single-cell clones of mutated cells (lanes 4-11) were detected in triplicate. The arrow indicates 707 bp fragments containing the conserved JEV E gene sequence. Only one single-cell clone, designated 3A10-3F, showed JEV RNA negative (lane 8). BHK-21 cells (lane 1) and BHK-21 cells infected with JEV (lane 2) were negative and positive controls respectively. Lane 3 was DL-2000 DNA marker.

Back to article page

Virology Journal

ISSN: 1743-422X