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Figure 1 | Virology Journal

Figure 1

From: A gastrointestinal rotavirus infection mouse model for immune modulation studies

Figure 1

Schematic design of the rotavirus double infection model. At day 0 the pups were born. From day 4 until day 10 after birth pups one group received intervention of rotavirus-specific antibodies (Gastrogard-R®) by oral gavage. At day 7 of age pups were inoculated with RRV by oral gavage. From day 8 on, the number of pups experiencing illness and the severity of illness was scored daily. From day 8 until the end of the experiment, feces were collected. At day 16 a blood sample was collected and at day 17 of age pups were inoculated with EDIM by oral gavage. At day 27 of age a rotavirus-specific DTH was elicited by subcutaneous inoculation of rotavirus in the ear pinnea, EDIM in the left ear and RRV in the right ear. DTH responses were measured 24 hours later, prior to collection of spleen and blood samples.

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