Figure 2
Specificity and sensitivity of RT-LAMP for the detection of IBDV. (A) Five related avian pathogens and vvIBDV Gx strain were subjected to RT-LAMP using the primers shown in Figure 1, and the RT-LAMP products were examined by both agarose gel electrophoresis (upper panel) and visually inspection for color changes (lower panel). Lanes M, DNA marker DL2000 (TaKaRa, China, with bands of 2000, 1000, 750, 500, 250 and 100 bp); 1, vvIBDV Gx strain; 2, avian influenza virus A/Chicken/Shandong/6/96 (H9N2) strain; 3, Newcastle disease virus La sota strain; 4, infectious bronchitis virus F strain; 5, Marek's disease virus CV1988 strain; 6, chicken anemia virus M9905 strain; 7, DEPC-treated water. (B) RNA standards in vitro transcribed and serially diluted were subjected to RT-LAMP and the RT-LAMP products were examined by both agarose gel electrophoresis (upper panel) and visually inspection for color changes (lower panel). Lanes M, DNA marker DL2000 (TaKaRa, China); 1-6, 2.8 × 105, 2.8 × 104, 2.8 × 103, 2.8 × 102, 2.8 × 101 and 2.8 × 10° copies of RNA, respectively; 7, DEPC-treated water.