Proteomic analysis of swine serum following highly virulent classical swine fever virus infection

Table 1 Proteins with at least 1.5-fold quantitative alteration in expression in serum from CSFV-infected pigs measured by 2-D DIGE and MALDI-ToF MS analysis

Spotno^.a	Identified proteins	GI no.^b	Ratio (I/U)^c	MM^d(kDa)/pI	M/S^e	Seq Cov (%)^f	Score^h	t-tes Pvalue
632	similar to RNA binding motif protein 15B	gi\| 126336349	-2.1	99/9.9	7/12	9	71	0.004
636	serotransferrin	gi\| 136192	1.5	79/6.9	7/14	15	81	0.003
808	MGF 505-3R	gi\| 162849409	1.6	12/9.2	5/16	52	75	0.005
827	vitamin D-binding protein	gi\| 51863317	-2.1	25/5.0	9/19	33	126	0.001
1036	retinol-binding protein 4	gi\| 47522930	1.7	23/5.4	6/14	31	82	0.004
1098	similar to thrombin inhibitor isoform 2	gi\| 119915930	-1.5	42/5.6	5/11	28	78	0.002

a) Labels of protein spots in Figure 1.
b) GI no. is MASCOT results of MALDI-ToF MS from the NCBInr database.
c) Relative protein expression in infected/uninfected samples.
d) MM, Molecular mass.
e) Number of mass values matched/Number of mass values searched.
f) Sequence coverage (%), the percentage of sequence covered versus the size of the matched protein.
h) Probability based Mowse score.

ISSN: 1743-422X