Figure 7

Analysis of symptoms after infection with combinations of wild type and mutant viruses, and identification of replicative forms. A), Plant showing synergistic symptomatology due to mixed infection with PHYVV CP- + PepGMV; B) and C), Southern blot of plants infected with PHYVV CP- + PepGMV; D), Plant showing symptoms caused by viral synergism after mixed infection with PepGMV CP- + PHYVV; E) and F), Southern blot of plants infected with PepGMV CP- + PHYVV; G), Synergism after mixed infection with PHYVV TrAP- + PepGMV; H) and I), Southern blot of plants infected with PHYVV TrAP- + PepGMV; J), mixed infection with PepGMV TrAP- + PHYVV leading to symptom recovery of pepper; K) and L), Southern blot of plants infected with PepGMV TrAP- + PHYVV. Southern blot lanes: B, mock-inoculated plant; H, PHYVV-infected plant; G, PepGMV-infected plant; M, plant infected with PHYVV + PepGMV mixture. Lanes 1 to 10, plants infected with mutants and wild type viruses. Gp lane, cloned PepGMV genome; Hp lane, cloned PHYVV genome. Membranes were initially hybridized with PHYVV IR probe and afterwards with PepGMV IR probe, after regeneration.