Figure 6

Inoculation of pepper plants with tripartite mixtures. A) and E), schematic representations of components of tripartite mixtures used for inoculation; B), symptoms observed on plants infected with the tripartite mixture PepGMV (A+B) + PHYVV A; C) and D), Southern blot of DNA extracted from plants infected with tripartite mixture PepGMV (A+B) + PHYVV A; F), symptoms observed on plants infected with tripartite mixture PHYVV (A+B) + PepGMV A; G) and H), Southern blot of DNA extracted from plants infected with tripartite mixture PHYVV (A+B) + PepGMV A. The viral probe used in Southern blot is indicated. Southern blot lanes: 1 kb, molecular weight marker. B, mock-inoculated plant; H, PHYVV-infected plant; G, PepGMV-infected plant; M, plant infected with PHYVV + PepGMV mixture. Lanes 1 to 10 correspond to plants infected with tripartite mixture. Lane Gp corresponds to the cloned PepGMV genome; lane Hp, cloned PHYVV genome. Southern blot membranes were initially hybridized with PHYVV IR probe and later with PepGMV IR probe, after being regenerated.