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Table 1 The oligonucleotides encoding full-length and truncated versions of the KKPGGPG motif

From: Identification of a conserved JEV serocomplex B-cell epitope by screening a phage-display peptide library with a mAb generated against West Nile virus capsid protein

Designations of oligonucleotides

The sequences of oligonucleotides

Coding motifs (designations)

Hp-1-F

5'-AATTCaagaaaccaggagggcccggcTAAG-3'

KKPGGPG (Hp-1)

Hp-1-R

5'-TCGACTTAgccgggccctcctggtttcttG-3'

 

Hp-2-F

5'-AATTCaaaccaggagggcccggcTAAG-3'

KPGGPG (Hp-2)

Hp-2-R

5'-TCGACTTAgccgggccctcctggtttG-3'

 

Hp-3-F

5'-AATTCaagaaaccaggagggcccTAAG-3'

KKPGGP (Hp-3)

Hp-3-R

5'-TCGACTTAgggccctcctggtttcttG-3'

 

Hp-4-F

5'-AATTCccaggagggcccggcTAAG-3'

PGGPG (Hp-4)

Hp-4-R

5'-TCGACTTAgccgggccctcctggG-3'

 

Hp-5-F

5'-AATTCaagaaaccaggagggTAAG-3'

KKPGG (Hp-5)

Hp-5-R

5'-TCGACTTAccctcctggtttcttG-3'

 
  1. Notes: Bases introduced to form the overhanging ends of EcoR I and Sal I after annealing the two complementary oligonucleotides are shown in capital letters; stop codons TAA are added.