Figure 5
From: Interactions of SARS Coronavirus Nucleocapsid Protein with the host cell proteasome subunit p42
Co-localization of SARS-CoV NP and p42 in 2BS cells. (A) SARS-CoV NP was expressed in 2BS cells transfected with recombinant -pcDNA3.0+SNP22b (left panel), 2BS cells transfected with pcDNA3.0 (right panel; negative control). (B) Proteasome subunit p42 in 2BS cells localized with anti-p42 antibody and anti-rabbit IgG-FITC conjugate, excitation at 488 nm (lane 1). SARS-CoV NP expressed in 2BS cells, localized using an anti-SARS-CoV NP mAb and anti-mouse IgG-TRITC conjugate, excitation at 568 nm (lane 2). Co-localization was detected by merging lanes 1 and 2 (lane 3).