Figure 3
From: Interactions of SARS Coronavirus Nucleocapsid Protein with the host cell proteasome subunit p42
Expression and identification of GST and the p42-GST fusion protein. (A) Lysate of E. coli BL21 containing the pGEX-5X-1-p42 vector, expressing p42-GST fusion protein without IPTG induction (lanes 1, 5) or induced by IPTG (lanes 2, 4). Lysate of E. coli BL21 containing the pGEX-5X-1 vector expressing GST with IPTG induction (lane 6) or without IPTG (lane 7). Molecular weight markers (lane 3). (B) Western blot analysis of p42-GST fusion protein and GST expression using an anti-GST mAb. Lysates of GST-expressing E. coli BL21 with IPTG induction (lanes 1, 2). Lysates of p42-GST fusion protein-expressing E. coli BL21 with IPTG induction (lanes 6, 7). Supernatants of E. coli BL21 expressing GST lysed using lysozyme (lane 3). Supernatants of E. coli BL21 expressing p42-GST fusion protein lysed by lysozyme (lane 5). Molecular weight markers (lane 4).