Figure 1
From: Interactions of SARS Coronavirus Nucleocapsid Protein with the host cell proteasome subunit p42
Expression and Identification of SNP22b (SARS-CoV NP). (A) Supernatants (lanes 1 and 10) and pellets (lanes 2 and 6) of E. coli BL21 containing the pET22b-SNP22b vector expressing SARS-CoV NP, without IPTG induction. Supernatants (lanes 3 and 7) and pellets (lanes 4 and 8) of E. coli BL21 containing the pET22b-SNP22b vector and expressing SARS-CoV NP, induced with IPTG. Pellets of E. coli BL21 containing pET22b induced with IPTG (lane 5). Molecular weight markers (lane 9). The position of SARS-CoV NP (~47 kDa) is indicated by an arrow. (B) Western blot analysis of SARS-CoV NP using an anti-SARS-CoV NP horse polyclonal antibody. Lysate of E. coli BL21 expressing SARS-CoV NP, induced by IPTG (lane 1). Lysate of E. coli BL21 containing pET22b induced by IPTG (lane 2). Molecular weight markers (lane 3). The position of SARS-CoV NP (~47 kDa) is indicated by an arrow.