Figure 3
From: Diametrically opposed effects of hypoxia and oxidative stress on two viral transactivators
Oxidative stress induced by menadione and paraquot decrease transactivation by EBNA1, but increase transactivation by Tat. (A) Transfected C33a cells were split 6 hours post-transfection into aliquots and exposed to the indicated concentrations of menadione, or paraquot (B) for an additional 18 hours, prior to reporter analysis. The inset legend indicates the columns corresponding to each effector/reporter combination. Control cells were vehicle treated. Transactivation is expressed as a percent of transactivation observed in the control cells. Immunoblots indicate that neither menadione (C), nor paraquot (D) altered the expression of EBNA1 or Tat. β-actin was used as a loading control. Asterisks indicate statistical significance by the Wilcoxon rank-sum test (p < 0.05) for treated samples compared to vehicle-treated controls.