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Table 4 Oligonucleotide primers designed for PRRSV amplification by conventional RT-PCR and one step real-time RT-PCR

From: The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus

  

Length

Primer

Sequence (5'-3')

Primer (bp)

Product (bp)

Nf

CCCGGGTTGAAAAGCCTCGTGT

22

228a

Nr

GGCTTCTCCGGGTTTTTCTTCCTA

24

 

371f

CCCGGGTTGAAAAGCCTCGTGT

22

371b

371r

TGTAACTTATCCTCCCTGAATCTG

24

 
  1. a Length of product amplified by one step real-time RT-PCR primer pair.
  2. bLength of product amplified by conventional RT-PCR primer pair.