Figure 5
From: Fusion to chicken C3d enhances the immunogenicity of the M2 protein of avian influenza virus
Titers of antibody against sM2 in BALB/c mouse serum detected by ELISA. Sixty BALB/c mice aged 6 weeks were divided into 6 (n = 10) groups. The mice in the six test groups were immunized thrice with GST-sM2, GST-C3d-sM2, GST-C3d-L1-sM2, GST-C3d-L1-C3d-sM2, GST-C3d-L1-C3d-L2-sM2 and GST-sM2+IFA, respectively. Blood samples were collected before each vaccination and two weeks after the final booster, and antibody against sM2 were detected by ELISA. Ab-positive cut-off values were set as mean + 2 SD of non-immunized sera. An ELISA Ab titer was expressed as the highest serum dilution giving a positive reaction. The titer values were showed in 100 (Log2X ± S).