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Figure 2 | Virology Journal

Figure 2

From: An efficient in vitro-inoculation method for Tomato yellow leaf curl virus

Figure 2

Detection of TYLCV DNA in tissue-cultured NS16 tomato plants after inoculation with the infectious TYLCV clone pBTY [JU]. (A) Agarose gel showing PCR products (450 bp) amplified with the primer pair TYMF/TYMR from DNA extracts of plants inoculated in vitro with TYLCV. Lanes 1-4: DNA extracts from plants inoculated with pCAMBIA1380 (negative control); Lanes 5-8: DNA extracts from plants inoculated with pBTY [JU]. C: pBTY [JU] plasmid (positive control). M: Low range DNA marker (Fermentas). (B) Agarose gel showing amplification products after rolling circle amplification (RCA) with DNA of plants inoculated with TYLCV in vitro. Lanes 1-2: DNA extracts from plants inoculated with pCAMBIA1380 (negative control); Lanes 3-4: DNA extracts from plants inoculated with pBTY [JU]. M: High range DNA marker (Fermentas). (C) Agarose gel showing TYLCV DNA after digestion of the RCA products with Nco I. Lanes 1-2: DNA extracts from plants inoculated with pCAMBIA1380 (negative control); Lanes 3-4: DNA extracts from plants inoculated with pBTY [JU]. M: High range DNA marker (Fermentas).

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