Identification of super-infected Aedes triseriatus mosquitoes collected as eggs from the field and partial characterization of the infecting La Crosse viruses

Table 5 Substitutions that differ in frequency between SI+ and previous isolates with Da > 0.10.

Genome			SI+	Prev. publ.	RSCU	RSCU
Region		Da	codon	Codon	SI+	Prev. publ.
M segment
5' noncoding
42	5'NC	0.143	A	G	-	-
G2
478	Val	0.143	GUA	GUG	0.254	0.210
658	Gln	0.143	CAA	CAG	0.540	0.460
697	Leu	0.143	UUG	UUA	0.233	0.300
NSm
991	Ile	0.143	AUU	AUA	0.287	0.544
1204	Leu	-0.027 - 0.357	CUA	CUU	0.177	0.116
1207	Phe	0.143	UUC	UUU	0.453	0.547
1288	Leu	0.143	UUA	UUG	0.300	0.233
1306	Phe	0.143	UUU	UUC	0.547	0.453
1327	Cys	0.143	UGU	UGC	0.472	0.528
G1
1501	Thr	0.143	ACU	ACC	0.316	0.173
1543	Pro	0.182	CCG	CCA	0.054	0.492
1639	Leu	0.143	CUG	CUA	0.114	0.177
1690	Arg	0.143	AGG	AGA	0.422	0.578
1951	Gly	0.143	GGA	GGG	0.288	0.114
1984	Leu	0.143	CUG	CUA	0.114	0.177
2002	His	0.143	CAC	CAU	0.354	0.646
2584	Gly	0.143	GGA	GGG	0.288	0.274
2929	Pro	0.182	CCA	CCG	0.492	0.054
3169	Lys	0.143	AAG	AAA	0.381	0.619
3214	His	0.143	CAC	CAU	0.354	0.646
3310	Gly	0.182	GGA	GGG	0.288	0.274
3754	Val	0.143	GUA	GUG	0.254	0.210
3916	Leu	0.143	UUA	UUG	0.300	0.233
4066	Asn	0.143	AAC	AAU	0.307	0.693
4156	Lys	0.143	AAG	AAA	0.381	0.619
3' noncoding
4395	3'NC	0.143	G	A	-	-
4430	3'NC	0.143	C	U	-	-
4482	3'NC	0.143	G	A	-	-
L segment
817	Ser	0.300	UCA	UCG	0.269	0.048
3' noncoding
6888	3'NC	0.300	U	C	-	-
Average					0.317	0.362
T-test [25 d.f.] prob.					0.130

All codon substitutions are synonymous. The codon used and the associated RSCU are listed for each substitution. Grey highlighted values differed by > 0.1 between the previously published and SI+ isolates. The average RSCU for previously published and SI+ isolates appears at the bottom of the table. The probability value is from a pair-wise t-test of differences in RSCU between SI+ and previously published isolates.

ISSN: 1743-422X