Influenza A viral loads in respiratory samples collected from patients infected with pandemic H1N1, seasonal H1N1 and H3N2 viruses

Table 1 Sequences of primers and probes for PCR and real time RT-PCR.

Primer and probe	Sequence (5'>3')	Reference
Bm-M-1	TAT TCG TCT CAG GGA GCA AAA GCA GGT AG	Hoffmann E et al.
Bm-M-1027R	ATA TCG TCT CGT ATT AGT AGA AAC AAG GTA GTT TTT	Hoffmann E et al.
FluA Forward	GAC CRA TCC TGT CAC CTC TGA C	CDC
FluA Reverse	AGG GCA TTY TGG ACA AAK CGT CTA	CDC
FluA Probe¹	TGC AGT CCT CGC TCA CTG GGC ACG	CDC
RnaseP Forward	AGA TTT GGA CCT GCG AGC G	CDC
RnaseP Reverse	GAG CGG CTG TCT CCA CAA GT	CDC
RnaseP Probe¹	TTC TGA CCT GAA GGC TCT GCG CG	CDC

¹TaqMan^® probes are labeled at the 5'-end with the reporter molecule 6-carboxyfluorescein (FAM) and with the quencher, Blackhole Quencher 1 (BHQ1) at the 3'-end.

ISSN: 1743-422X