Monitoring human cytomegalovirus infection with nested PCR: comparison of positive rates in plasma and leukocytes and with quantitative PCR

Table 2 Sensitivity, specificity and predictive values of nested PCR with plasma and qRT-PCR with PBL

Process	Sensitivity (%)	Specificity (%)	Positive predictive value (%)	Negative predictive value (%)	Concordance to nested PCR with PBL (%)
qRT-PCR with PBL	35.1	100	100	74.2	77.4
Nested PCR with plasma	48.6	97.1	90.0	77.9	80.2
Nested PCR with PBL^a	100	100	100	100	--

^aNested PCR with PBL was considered as the reference technique in detecting HCMV DNA.

ISSN: 1743-422X