Figure 2

Two representative agarose gels of PCR products demonstrating different HCV genotypes. Plasma samples from patients positive for HCV nucleic acid were subjected to genotype determination using a specific RT-PCR kit which produces different size PCR products related to different genotypes. A) Lanes 1, 6 and 11: 100 bp DNA size marker, lane 2: positive control for genotype 1a (338 bp), lane 3: positive control for genotype 1b (395 bp), lane 4: positive control for genotype 2 (286 bp), lane 5: positive control for genotype 3a (227 bp), lanes 7 and 8: two sets of PCR amplifications for one patient resulting in genotype 3a, lanes 9 and 10: two sets of PCR amplifications for another patient resulting in genotype 1a. B) Lanes 1, 6 and 12: 100 bp DNA size marker, lane 2: positive control for genotype 1a (338 bp), lane 3: positive control for genotype 1b (395 bp), lane 4: positive control for genotype 2 (286 bp), lane 5: positive control for genotype 3a (227 bp), lanes 7 and 8: two sets of PCR amplifications for one patient resulting in genotype 1a, lane 9: empty well, lane 10: patient positive for genotype 2, lane 11: patients positive for genotype 1b.