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Figure 4 | Virology Journal

Figure 4

From: Characterization of the HIV-1 integrase chromatin- and LEDGF/p75-binding abilities by mutagenic analysis within the catalytic core domain of integrase

Figure 4

Subcellular localization of IN 170 EHLK 173 mutants in COS-7 cells. COS-7 cells were transfected with different CMV-YFP-IN fusion protein expressors as indicated for 48 h. After fixation and permeabilization, cells were incubated with primary rabbit anti-GFP antibody followed by secondary FITC-conjugated anti-rabbit antibodies, and the nuclei were stained with DAPI. Cells were visualized by a Carl Zeiss microscopy (Axiovert 200) with a 63× oil immersion objective.

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