Figure 3

Construction of expression plasmids. The expression plasmid for the chimeric envelope was assembled by cloning HBV preS2 and ENV coding sequences to multiple cloning sites of the eukaryotic expression plasmid pcDNA3.1(-). The insertion sites are Hind III and Xho I in mutated ENV. The pGAG-POL and pRz recons expressed Moloney murine leukemia virus (Mo-MLV), Gag-Pol protein, and HDV ribozyme, respectively. The ENV and GAG-POL expression plasmid contains an MoMLV CMV-driven promoter, while Rz is an MoMLV LTR promoter. All plasmids contain SV40 origins of replication. SP, signal peptide.