Immunohistochemical analysis of HBsAg and HBcAg in liver tissues.(A) Strong HBsAg immune positivity was shown in hepatocytes (arrow). (B) Immunopositivity for HBsAg was mainly distributed in cytoplasm of hepatocytes. (C, D) HBcAg was distributed mainly distributed nucleus of hepatocytes. Spotty parenchymal cell degeneration, with necrosis and karyopyknosis (arrow) of hepatocytes were observed. Original magnification×400. (A, C: liver sample from CP74; B, D: liver sample from DX385).