Figure 3From: Highly pathogenic avian influenza A virus H5N1 NS1 protein induces caspase-dependent apoptosis in human alveolar basal epithelial cellsCaspase-3 and caspase-9 activation in NS1-transfected A549 cells. The cells were transfected with the plasmid pCMV-Myc/NS1 for 0 h, 12 h, 24 h, and 48 h. Intracellular caspase-3 and caspase-9 activation were detected by Western blotting. The β-actin was used as a loading control.Back to article page