Figure 4
From: Species-independent bioassay for sensitive quantification of antiviral type I interferons
RFVFV-Ren antiviral assay on cells lacking the type I IFN receptor. Murine L929 cells were infected with Clone 13 at an MOI of 1, or left uninfected (mock). After 16 h of infection, supernatants were harvested and sterilized as described for Fig. 2B. (A) Determination of IFN-β using a commercial ELISA. Mean values from two measurements are shown. (B) Analysis for inhibition of RVFV-Ren on IFNAR-/- MEFs. Different doses of recombinant pan-species IFN-α were used as control. Measurements were performed in triplicate; columns show means ± SD.