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Figure 3 | Virology Journal

Figure 3

From: Species-independent bioassay for sensitive quantification of antiviral type I interferons

Figure 3

Dose-response curves for mouse and chicken IFNs. Murine L929 cells (A) or CEFs (B) seeded in 96-well plates were pre-treated for seven hours with increasing doses of pan-species IFN-α and chicken IFN (chIFN), respectively. Both assays were then carried out further as described for Fig. 1B. All measurements were performed in triplicate wells under standard conditions; shown are means ± SD. Both axes are plotted in logarithmic scale; the dotted vertical line indicates the threshold of sensitivity, based on the mean + 2SD of an untreated control run in triplicate; shaded area shows the linear range of the assay.

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