Table 1 Primers used for the amplification of genes analysed in this study.
From: Molecular characterisation of virulence graded field isolates of myxoma virus
Gene | Gene descriptiona | Forward primer (5'-3') and genomic positionb | Reverse primer (5'-3') and genomic positionb | Amplicon size (bp) | Reason for inclusion |
|---|---|---|---|---|---|
M002L/R | TNF receptor homolog | GGTCCGTGATTAATATTCG 2864 - 2882/158892-158910(c) | GAATTCCACGCTGATGTAG 2121-2139/159635-159653(c) | 761 | Mutations detected in field strains [6] |
M004L/R | ER localized apoptosis regulator | GGAATCTAGATAAGGAACATTG 4271-4292/157482-157503(c) | CGTCTTCCCGTAGAAGTC 5001-5018/156756-156773(c) | 747 | RFLP (data not shown) |
M004.1L/R | Unknown | ||||
M005L/R | Ankyrin-like | ||||
M007L/R | IFNγreceptor homolog | CGAGGGTGATGTCGCTCATG 7891-7910/153864-153883(c) | GCGTACGCCACCTACCTCG 8835-8853/152921-152939(c) | 962 | Virulence factor [20] |
M009L | Kelch ring canal protein homolog | CGCAGGTCCACGTATAAACC 11482-11501 | ACGTGGGAAGCCGATGTC AG 13413-13432 | 1950 | Mutation detected in strain 6918 [8] |
M010L | EGF-like growth Factor | CGTTATGTGTACCGTATATG 13291-13310 | GCGTGGGCACGTTGAACA CG 14180-14199 | 908 | |
M011L | Integral membrane protein apoptosis regulator | ||||
M017L | Unknown | CGTAGTAGTCAGAATCGTCG 17932-17951 | CTATCCGGAAATCATAACC 18337-18355 | 423 | Mutations detected in field strains [6] |
M034L | DNA polymerase | GAACGAGGTTCGCATTGTAC 35048-35067 | CTCTCAGGTGTTGAGTACG G 35691-35710 | 662 | Potential diagnostic PCR |
M036L | Leucine zipper motif | CAATCTAGCGTCAGATCCC 37128-37146 | GTTTTCTGCGGATGGATCT C 39333-39352 | 2224 | Mutation detected in strain 6918 [8] |
M069L | Tyrosine/serine phoshatase | GAACTTAGAGTTGCTCATGCG 65999-66019 | GAAGAAACAGACCGTGGA CAC 66714-66734 | 735 | Mutation detected in strain 6918 [8] |
M071L | Immunodominant envelope protein | CCATTGACTAACTCTGTTCC 67391-67410 | CTAAATGGCGTCTCCTAGC 68165-68183 | 792 | |
M121R/M122R | EEV glycoproteins | CAGACATCATGTCGTTACAC 115841-115860 | CTTATAGAATCTTTTCATA C 116882-116901 | 1060 | Gene function [12] |
M130R | Virulence factor | GAGGATCATCCGAAGGAG 122677-122694 | GGACTGTATATATCGCCTC 123373-123391 | 714 | Virulence factor [21] |
M135R | IL-1/IL-6 receptor-like | CCTACGTGTTTACTAGATTACG 131566-131587 | GATTATCCTTCGTACGTCG 132290-132308 | 742 | |
M141R | Immunoglobulin domain | GAGAGACGATGCGTGTGTTAAG 137061-137082 | GATGCATCGATTAACACGT C 137761-137780 | 719 | Virulence factor [12] |
M144R | Complement control protein homolog | CGTATCGGTTACGAAGAGTAG 139330-139350 | CTAGATCGCCTCCTCTCCA GC 140369-140389 | 1059 | Gene function [12] |
M148R | Ankyrin-like | GCAAGCCGATGAGTTACTC 141533-141551 | CGAATCCAGATTGTAGTAG 143727-143745 | 2212 |