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Table 2 Primers and probe used in this study

From: A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes

Name

Sequencea

Nucleotideb

Sense

5'-TTGGTTACCATGCAAACAAYT-3'

91-111

Antisense

5'-TRTCTTGGGCRTGTGTAACA-3

152-171

Probe

5'-FAM-CAGGTTGA CA CAA TAA TGGAAAA G-BHQ3-3'

119-143

  1. a Y = T or C, R = A or G. LNA residues in the probe are indicated in bold. 5' - FAM = 5' - carboxyfluorescein, BHQ = Black Hole Quencher. b The position in the HA gene is indicated.
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Virology Journal

ISSN: 1743-422X