Figure 4
HBx increases cell invasion ability. Matrigel invasion assay of HepG2-pcDNA3 and HepG2-pcDNA3-X cells. Cells (1 × 106) were resuspended in conditioned medium and added to the upper compartments of matrigel invasion chambers supplemented with or without 5 mM 10058-F4 for 24 h. The total number of cells that invaded to the underside of the filters was counted. The values obtained were calculated by averaging the total number of cells from three filters. As a control, a parallel experiment was performed using the device with uncoated membrane. The results represent means of triplicates; * statistically different from control at P < 0.05.