Figure 5

Rabbit serum binds to two proteins (38 kDa and 95 kDa) on teleost cells. BGMK, BF-2, and FHM cells were harvested and run on a 10% SDS acrylamide gel. Proteins were transferred from the gel to a PVDF membrane. The membrane was probed with (A) rabbit pre-immune serum, (B) rabbit pre-immune serum pre-incubated with protein A, or (C) no primary serum was added. Proteins were then visualized using peroxidase conjugated goat anti-rabbit IgG and chemiluminescence.