Figure 3From: Antibody dependent enhancement of frog virus 3 infectionAddition of rabbit pre-immune serum to BF-2 cells inhibits ADE. FV3 (~50 PFU) was incubated with either 50 ng of rabbit anti-FV3 serum or 50 ng rabbit pre-immune serum. FV3+anti-FV3 serum or FV3+pre-immune serum complexes were added to BF-2 cells along with varying amounts of pre-immune serum (0-1000 ng). Cells were overlaid and plaques were visualized with crystal violet. Mean plaques values from 3 independent trials were obtained and mean values were expressed as a relative percentage to BF-2 cells infected with FV3 in the absence of anti-FV3 serum or pre-immune serum.Back to article page