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Figure 1 | Virology Journal

Figure 1

From: Antibody dependent enhancement of frog virus 3 infection

Figure 1

ADE occurs during an FV3 infection in teleost cells. FV3 (~50 PFU) was incubated alone, with rabbit anti-FV3 serum, or rabbit pre-immune serum for 1 hour at 4°C and was then added to BF-2 or FHM cells. (A) Two hours post-infection, BF-2 cells were overlaid and 48 hours later the plaques were stained with crystal violet and were counted. Plaque numbers are shown as a relative percentage of a control FV3 infection in the absence of serum. (B) Forty-eight hours post-overlay BF-2 cells were stained by indirect immunofluorescence using anti-FV3 serum (green) and DAPI (nuclei - blue). (C) FV3+anti-FV3 serum or FV3+rabbit pre-immune serum complexes were added to FHM cells and 24 hours later plaques were visualized by indirect immunofluorescence and counted. Plaque numbers were expressed as a relative percentage compared to FHM cells infected with FV3 only. All experiments were completed in 3 independent trials and mean plaque numbers are shown.

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