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Table 1 Effects of formaldehyde and glutaraldehyde on infectivity of HCVcc in culture medium

From: Thermal stability and inactivation of hepatitis C virus grown in cell culture

Treatment

Infectious titer

(FFU/ml)

IFA result

  

2nd cell passage

3rd cell passage

Formaldehyde (0.037%)

   

   0 h

(3.9 ± 0.3) × 104a

NTc

NT

   0.5 h

(1.1 ± 0.1) × 104

NT

NT

   1.0 h

(4.0 ± 1.0) × 103

NT

NT

   1.5 h

(1.7 ± 0.6) × 103

NT

NT

   2.0 h

≤ 1.0 × 103b

positive

NT

   2.5 h

< 1.0 × 103

positive

NT

   3.0 h

< 1.0 × 103

negative

negative

Glutaraldehyde (0.01%)

   

   0 min

(4.0 ± 0.3) × 104a

NT

NT

   10 min

(3.3 ± 0.6) × 103

NT

NT

   20 min

< 1.0 × 103

negative

negative

  1. a Untreated viral samples were retitrated in culture medium containing 0.00037% formaldehyde or 0.0001% glutaraldehyde as controls.
  2. bOne focus was detected in one of the triplicate wells in FFU assay.
  3. c NT, not tested. Samples with positive results in FFU assay or at the second cell passage were not tested for NS3 staining for the third cell passage.