Detection and subcellular localization of the GFP-NS2 and NS2-GFP fusion protein expressed in SUVEC. (A) Western immunoblot using anti-GFP antibody of cellular proteins isolated from various cell lines. (B) Confocal microscopy images of SUVEC cells. All the cell lines were stained by Hoechst33342 and ER-Tracker™ Red. Merged images show co-localization of GFP, GFP-NS2 and NS2-GFP in the ER. Bar = 20 μm for all the figures.